Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes

Figure 3

Effect of MG exposure on glyoxalase 1 activity and expression in astrocytes and C6 cultures. Rat primary astrocyte cultures and C6 glioma cells were exposed to 0.4 or 1 mM MG at 24 h. Glyoxalase 1 activity (a, b) and expression (c, d). Each value represents the mean ± standard error of five independent experiments performed in triplicate from the glyoxalase 1 activity assay and three independent experiments performed in duplicate from Western blot analysis in both cell cultures. Control value is assumed as 100%. Insets are representative immunoblots for glyoxalase 1 (GLO1) or actin. Data were analyzed by one-way ANOVA followed by Dunnett’s test, assuming . Significantly different from control.

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