Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes

Figure 4

GSH content in astrocytes and C6 glioma cells after MG incubation. Rat primary astrocyte cultures and C6 glioma cells were exposed to 0.4 or 1 mM MG at 24 h. GSH content (a, b) is shown. Each value represents the mean ± standard error of six independent experiments performed in triplicate, assuming the control value as 100% for each type of cell culture. The mean absolute values of GSH in the control cell cultures were 38 and 39 nmol/mg protein in astrocytes and in C6 cells, respectively. Data were analyzed by one-way ANOVA followed by Dunnett’s test, assuming . Significantly different from control.

(a)

(b)