Methylglyoxal Induces Changes in the Glyoxalase System and Impairs Glutamate Uptake Activity in Primary Astrocytes

Table 1

AG blocks the increments in glyoxalase 1 activity and GSH content following 1 mM MG.

	Astrocytes
Treatment	Glyoxalase 1 activity	GSH content
Control	100.0 ± 5.0	100.0 ± 16.7
MG	123.6 ± 2.0	182.7 ± 33.3
AG	109.2 ± 3.4	80.9 ± 15.5
MG + AG	106.9 ± 3.4	93.6 ± 3.5

Rat primary astrocyte cultures were exposed to 1 mM MG and 0.4 mM AG at 24 h. Percentages of glyoxalase 1 activity and GSH content are shown. Each value represents mean ± standard error of five independent experiments performed in triplicate, assuming the control value as 100%. Data were analyzed by one-way ANOVA followed by Dunnett’s test, assuming . Significantly different from control.