Vialinin A, an Edible Mushroom-Derived p-Terphenyl Antioxidant, Prevents VEGF-Induced Neovascularization In Vitro and In Vivo

Figure 5

Effect of vialinin A on NF-κB activation. Growth-arrested HUVECs were pretreated with vialinin A (5 μM) overnight followed by treatment with VEGF for the indicated time periods. (a) Equal amounts of nuclear extracts were subjected to Western blot analysis using phospho-p65 antibodies. A representative blot is shown (). (b) NF-κB DNA binding activity was determined by using a NF-κB transcription factor assay kit from Cayman Chemicals. Data were presented as the mean ± SD (). when compared to untreated control; ^## when compared to VEGF treated.

(a)

(b)