Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

Figure 4

Role of the AMPK-SIRT1 signaling pathway in KGN-mediated antioxidant effect. (a) The mRNA levels of SIRT1 in KGN-treated BM-MSCs were measured using real-time RT-PCR. Values are the of four independent experiments () in real-time RT-PCR experiments. (b) The protein levels of SIRT1 were determined using Western blot assays. (c) Quantification of protein levels of SIRT1. (d) Activation of AMPK by KGN was determined using Western blot assays. (e–f) The phosphorylated levels of AMPK (e) and total expression of AMPK (f) in KGN-treated BM-MSCs were quantified. Values are the of four independent experiments () in real-time RT-PCR experiments and of three independent experiments () in Western blot assays. Statistically significant differences are indicated by where between the indicated groups and where versus the CTRL group.

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