Research Article

NLRP3 Inflammasome Formation and Activation in Nonalcoholic Steatohepatitis: Therapeutic Target for Antimetabolic Syndrome Remedy FTZ

Figure 6

Nlrp3 gene silencing inhibited PA-induced NLRP3 inflammasome production and steatosis in HSCs with and without FTZ treatment. (a) Representative oil red O images showing positive staining of lipid deposition in PA-treated HSCs, which was suppressed by Nlrp3 siRNA and FTZ. (b) Staining area of lipid deposition showing a statistically significant increase induced by PA, which was reduced by Nlrp3 siRNA and FTZ. (c) ELISA of IL-1β levels from cell media showing a statistically significant decreased by Nlrp3 siRNA and with FTZ ( batches of cells). versus Ctrl-scram group; versus PA-scram group.
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