Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway

Figure 4

Oryzanol activates the transcription of Nrf2 target genes. HEK-293 cells were pretreated with 5 μg/mL ORY for 24 h followed by a time course of 100 μM H₂O₂ (1, 3, 6, and 12 h). Cells were then processed for measuring Nrf2 (a), Keap1 (b), HO-1 (c), and NQO1 (d) mRNA levels by real-time PCR. GAPDH was used to normalize the results. Data are shown as mean ± SEM. Statistically significant differences were represented as follows: , , , and versus untreated cells and and versus the corresponding H₂O₂ control group, Bonferroni’s multiple comparison test.

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