GRP78 Promotes Neural Stem Cell Antiapoptosis and Survival in Response to Oxygen-Glucose Deprivation (OGD)/Reoxygenation through PI3K/Akt, ERK1/2, and NF-κB/p65 Pathways

Figure 4

Role of GRP78 in OGD/reoxygenation-induced NF-κB/p65 activations. (a, b) Abolished phosphorylation of IKK, IκB, and p65 by OGD+R induction in GRP78 knocked-down NSCs. GAPDH was used as an internal loading control in whole cell lysate protein expression; H3 was used as an internal loading control in nuclear protein expression. (c, d) Abolished nuclear translocation of phospho-p65 by OGD+R induction in GRP78 knocked-down NSCs. and was considered to be significantly different in Neg-siRNA-control versus Neg-siRNA-OGD+R, Neg-siRNA-control versus GRP78-siRNA-control, and GRP78-siRNA-control and GRP78-siRNA-OGD+R groups. . Scale bar: 20 μm.

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