Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion

Figure 4

Sulfide inhibits lipid-peroxidation in vivo and in vitro. (a, left panel) Representative oil red O staining of aorta derived from ApoE^−/− mice fed with atherogenic diet and treated with NaSH (), PPG (), or vehicle (PBS, ) for 8 weeks. (a, right panel) Quantification of atherosclerotic plaque size was performed with ImageJ software. (b, left panel) Representative aortic sections (6 μm) stained for F-actin (phalloidin-FITC, green), 4-HNE (red), and DNA (Hoechst, blue) (). (b, right panel) TBARS content of aorta derived from ApoE^−/− mice fed with atherogenic diet and treated with vehicle () or NaSH for 8 weeks (). (c) ApoE^−/− mice were fed with regular diet () or atherogenic diet for 8 weeks. Parallel with the atherogenic diet, the mice were kept intraperitoneally vehicle () or NaSH (). Immunohistology was showing hematoxylin and eosin and HO-1 staining of mice aorta samples. Endothelial cells are indicated by arrows, smooth muscle cells were marked by cross, and macrophages were marked by comparison signs. The magnification of the mice aortas was 700x. (d) Real-time qPCR was showing HO-1 relative expression in mouse aorta. indicates statistical significance (). indicates statistical significance ().

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