Effect of the Antioxidant Lipoic Acid in Aortic Phenotype in a Marfan Syndrome Mouse Model

Figure 8

Quantitative analysis of microfluorotopography of DHE oxidation products in the (a) aortic arch, (b) thoracic aorta, and (c) abdominal aorta of wild-type (WT) and Marfan syndrome (MFS) mice untreated or treated with losartan or lipoic acid. (d) Representative photomicrographs of six-month-old wild-type (WT) and Marfan syndrome (MFS) mice, showing microfluorotopography of DHE oxidation products. Red staining indicates the fluorescence signal by DHE under 400x magnification. Bars: 50 μm. Quantitative analysis of DMPO-protein radical adduct detection in the (e) aortic arch, (f) thoracic aorta, and (g) abdominal aorta of 6-month-old wild-type (WT) and Marfan syndrome (MFS) mice. (h) Representative photomicrographs of six-month-old wild-type (WT) and Marfan syndrome (MFS) mice, showing DMPO-protein radical adduct detection in the aortic arch and thoracic and abdominal aortas segments. Green staining indicates the fluorescence signal by DMPO, and blue stain (DAPI) indicates the nuclei under 400x magnification. Bars: 50 μm. ^a versus WT untreated; ^b versus MFS untreated; ^c versus WT losartan treatment; ^d versus MFS untreated; ^e versus MFS losartan treatment; ^f versus WT untreated; ^g versus WT losartan treatment; ^h versus WT untreated; ⁱ versus MFS untreated; ^j versus WT losartan treatment; ^k versus MFS losartan treatment. Data expressed in mean ± SEM.

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