SGK1 Inhibits Autophagy in Murine Muscle Tissue

Figure 6

Elevated LC3 lipidation in SGK1-depleted cells. Stable GFP-WIPI1 expressing U-2 OS cells were transiently transfected with control siRNA (siControl) or siSGK1 and fed or starved for 3 h with or without bafilomycin A1 (BafA1). (a) SGK1 depletion was confirmed by Western blotting (, 3 independent experiments are shown). The autophagic flux was assessed by LC3 (LC3-II) Western blot analysis. (b) LC3-II levels were normalized over tubulin (, 3 experiments are shown). values are provided. Supporting data is provided (Suppl. Data Set).

(a)

(b)