Sulforaphane Modulates AQP8-Linked Redox Signalling in Leukemia Cells

Figure 3

Effect of SFN on AQP8 content in plasma membrane of B1647 cell line. Representative confocal images of B1647 cells treated (SFN) or not (CTRL) with 10 μM sulforaphane for 24 h and labelled with DAPI (blue) and anti-aquaporin 8, AQP8, (green). Cells were not permeabilized in order to exclude intracellular signals. Scale bar = 10 μm. Triple magnification of representative superimposed 3 central slices is shown in white squares. Images were acquired by Nikon A1 confocal laser scanning microscope (Nikon Instruments, Japan). The results are the representative of two independent experiments.