Nucleoredoxin-Dependent Targets and Processes in Neuronal Cells

Figure 3

Classification of potential Nrx interaction partners identified by mass spectrometric analysis. To identify potential Nrx interaction partners and targets, three mass spectrometric-based approaches were performed, namely, a differential labeling with neuroblastoma SH-SY5Y cells lacking Nrx as well as two intermediate trappings (IT) with recombinant mNrx Cys208Ser using SH-SY5Y cell and mouse brain extracts. The results from the differential labeling were used to analyze the redox proteome of Nrx-depleted cells revealing that 94.2% of the identified cysteine-containing proteins were more reduced in the absence of Nrx (a). The potential interaction partners of Nrx, identified by all three approaches, were compared regarding their biological processes (b) and their molecular function (c) using the PANTHER classification system. There is a big resemblance between the results of the three approaches, with most potential interaction partners having catalytic activities and functions in binding, as well as metabolic and cellular processes.

(a)

(b)

(c)