Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice

Figure 3

Effects of bacterial thioredoxin (Trx) in body temperature and weight, organ failure, and survival of SIRS mice. Bacterial Trx (20 μg/150 μl/mouse, subcutaneously, twice a day, for 3 days prior to lipopolysaccharide (LPS) injection) effects in (a) body weight and (c) temperature of TRPC5^+/+ and TRPC5^−/− mice injected with vehicle (PBS; ) or LPS (). Effects of bacterial Trx (20 μg/150 μl/mouse, subcutaneously, twice a day, for 3 days prior to LPS injection) in (b) body weight and (d) temperature, (e) AST, and (f) creatinine levels in SIRS mice treated with vehicle (6% DMSO in PBS; ) or the TRPC4/TRPC5 antagonist ML204 (1 mg/kg; ) subcutaneously, twice a day, for 5 days prior to LPS injection. Non-SIRS (PBS-injected) mice were used as controls (). Survival rates (e) were registered for mice administered with vehicle, ML204, Trx, or Trx + ML204 (/group). differs from vehicle-injected controls; differs from LPS-injected controls.

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