Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages

Figure 3

Candida albicans treatment induces LAP. (a) THP-1-derived macrophages were pretreated with GFP-LC3 and stimulated with C. albicans (CFW stained) for 8 hours. CQ-treated THP-1-derived macrophages showed LC3 and p62 colocalization and gathering into clusters. In C. albicans-treated cells, C. albicans spores colocalized with LC3 but without p62. (b) THP-1-derived macrophages were stimulated with C. albicans (CFW stained) for 8 hours. CQ-treated THP-1-derived macrophages harboured LC3 clusters, and the expression level of ATG9A increased. In C. albicans-treated cells, C. albicans spores colocalized with LC3 but without ATG9A. (c, d) BMDMs were treated with live C. albicans spores for 8 hours. The colocalization of Lyso-tracker and LC3 or Lyso-tracker and Rubicon was detected.

(a)

(b)

(c)

(d)