A Novel Small Peptide Inhibitor of NFκB, RH10, Blocks Oxidative Stress-Dependent Phenotypes in Cancer

Figure 3

RH10 affects ROS production and angiogenesis in vitro. (a) KAT-4 cells were plated at a density of 5 × 10⁴ for each well in 24-well plates. After incubation with the peptide RH10 or the negative control for 24 h, the cells were incubated with 5uMH₂DCFDA for 30 minutes, and fluorescence was immediately measured at a plate reader. RH10 was able to reduce ROS production compared with controls. Data are reported as mean ± SD ( vs. CTRL; vs. CTRL−). (b) VEGF gene expression was evaluated by real-time PCR. RH10 reduced VEGF expression compared with controls. Data are reported as mean ± SD ( vs. CTRL; vs. CTRL−). (c–d) Endothelial cells were plated on Matrigel matrix, and tubular formation was evaluated 24 hours after treatment with CTRL− or RH10. RH10 inhibits angiogenesis in vitro compared with control and CTRL−(c). In another set of experiments, endothelial cells were treated with supernatants from KAT-4 cells treated with peptides (d). RH10 was able to inhibit tumor angiogenesis. The images are the representative of the results from the three independent experiments.

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