p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke

Figure 2

CSE increased p66Shc expression in a time-dependent manner in Beas-2b cells. Cell viabilities were determined by MTT and trypan blue exclusion assay, and they were gradually decreased when airway epithelial cells were incubated with 7.5% CSE for 0–48 h (a). After stimulation with 7.5% CSE at the indicated time points, real-time PCR showed that p66Shc mRNA expression was increased in a time-dependent manner (b). Western blot showed that the expression levels of p66Shc and p-p66Shc in the whole cell lysates (c) and p66Shc expression in the mitochondrial lysates (d) were all increased in a time-dependent manner when the cells were stimulated with 7.5% CSE at the indicated time points. All statistical data were obtained from three independent experiments and presented as the mean ± SD. compared to the cells treated with CSE for 0 h.

(a)

(b)

(c)

(d)