The Effects of Ibogaine on Uterine Smooth Muscle Contractions: Relation to the Activity of Antioxidant Enzymes

Figure 4

Protein levels of antioxidant enzymes in the uterus after treatment with increasing concentrations of ibogaine. (a) Protein levels of antioxidant enzymes in spontaneously active uteri (representative Western blots are shown underneath the corresponding charts). (b) Protein levels of antioxidant enzymes in Ca²⁺-stimulated uteri (representative Western blots are shown underneath the corresponding charts). Whole cell extracts from uteri (50 μg protein) were subjected to SDS-PAGE and Western blotting. β-Actin was used as loading control. Representative Western blots and relative quantification of antioxidant enzyme levels of control uteri (C). Uteri treated with increasing concentrations of ibogaine up to both 20 mg/l and 40 mg/l are shown. Values are means ± SEM () and are presented as percentage of control.

(a)

(b)