Research Article

Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting

Figure 1

Characterization of an in vitro and in vivo model of cancer-associated atrophy. C2C12 myotubes treated for 48 h with 10% conditioned medium (CM) from KPC cells. Dexamethasone (DEXA) was used as a positive control of atrophy induction. Pictures were acquired at the brightfield microscope and (a) diameters were measured. (b) Increased ROS production in C2C12 myotubes treated with CM from KPC using oxidant-sensitive fluorescent dye H2DCFDA. (c) No difference in food intake in tumor-bearing versus control mice at the end of the experiment. (d) No significant weight loss in KPC tumor-bearing versus control mice. (e) Average tumor weight (min max box plot). Upon tumor growth at the end stage, mice presented reduced muscular resistance as evidenced by (f) performance at the hanging test (reach/fall assay) and (g) maximal strength as performed by the grip test of the upper limbs. All experiments have been performed with N ≥ 4 mice per group. All data are shown as means ± SEM; n.s. = nonsignificant; ; ; one-way ANOVA with Bonferroni correction for (a), two-way ANOVA with Dunnett’s multiple comparison test for (e), and Student’s t-test for (b), (c), (d), (f), and (g).
(a)
(b)
(c)
(d)
(e)
(f)
(g)