Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting

Figure 3

Metabolic dysregulation of skeletal muscle in KPC-bearing mice. (a) Representative images for succinate dehydrogenase (SDH) activity stain. (b) Gastrocnemius mitochondria were isolated, and ETC activity from complex I to complex III was assessed by evaluating cytochrome c reduction. (c) ¹⁴C-labeled palmitate was used as a substrate to measure lipid beta-oxidation in isolated mitochondria of gastrocnemius from control and KPC-bearing mice. (d) Representative T₁-weighted MR images (brightest regions in T₁-weighted MR images correspond to adipose regions) and (e) in vivo measurement of adipose tissue using MR images in the control group versus KPC-bearing mice. (f) Weight of inguinal fat normalized on tibial length for control versus KPC-bearing mice. (g) Gastrocnemius mitochondria were isolated, and ATP level was assessed using the ATPlite kit (PerkinElmer, USA). (h) ROS measurement in isolated mitochondria from gastrocnemius using H₂DCFDA. N ≥ 4. All data are shown as means ± SEM. Statistical analysis was conducted using two-tailed t-test. n.s. = nonsignificant; ; .

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