<i>β</i>3-Adrenoreceptors Control Mitochondrial Dormancy in Melanoma and Embryonic Stem Cells

<div><i>β</i>3-AR/UCP2 axis blockade affects the glycolytic metabolism of both A375 and ES cells. Western blot analysis of UCP2 marker after treatment with BRL37344 (10 <i>μ</i>M) + genipin (15 <i>μ</i>M), BRL37344 (10 <i>μ</i>M), and BRL37344 (10 <i>μ</i>M) + SR59230A (10 <i>μ</i>M) (a, e). Glucose uptake and lactate export assays (b, f). WB analysis of HKII and MCT-4 markers (c, g). ATP production assay (d, h). Data are representative of at least three experiments (mean and SD). <svg height="8.685721pt" id="M4" style="vertical-align:-0.04981613pt" version="1.1" viewbox="-0.049816 -8.635905 8.150709 8.685721" width="8.150709pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g></svg> values for SR59230A treatment <span class="nowrap"><svg height="10.739542pt" id="M5" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.049816 -10.102542 50.918385 10.739542" width="50.918385pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g 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236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,5.581,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,11.162,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 158Z"></path></g><g transform="matrix(.013,0,0,-0.013,17.241,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g transform="matrix(.013,0,0,-0.013,28.924,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g 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d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>.</span> <svg height="8.685721pt" id="M8" style="vertical-align:-0.04981613pt" version="1.1" viewbox="-0.049816 -8.635905 8.150709 8.685721" width="8.150709pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g></svg> values for genipin treatment: <span class="nowrap"><svg height="12.164466pt" id="M9" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.049816 -11.527466 49.785761 12.164466" width="49.785761pt" 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: <i>β</i>3-Adrenoreceptors Control Mitochondrial Dormancy in Melanoma and Embryonic Stem Cells 

Oxidative Medicine and Cellular Longevity

β3-Adrenoreceptors Control Mitochondrial Dormancy in Melanoma and Embryonic Stem Cells

Figure 2