Apigenin Protects the Brain against Ischemia/Reperfusion Injury via Caveolin-1/VEGF In Vitro and In Vivo

Figure 1

The effect of apigenin on cell viability of HBMVECs with OGD/R was explored. To make OGD/R, HBMVECs were cultured in conditions of oxygen deprivation, 1% O₂ +  5% CO₂ + 94% N₂ for 4 h, 6 h, 8 h, and 12 h and glucose-free culture, then reoxygenated for 24 h or 48 h in a complete medium, and cell viability was measured using CCK8 (a), Caveolin-1 mRNA expression was detected by qPCR (b), and western blotting was used to assay Caveolin-1 protein expression (c). Cell viability of HBMVECs during OGD/R treated with 2.5, 5, and 10 μM of apigenin were measured by CCK8 (d). Data was given as mean ± SD, . and compared with OGD; and compared with reoxygenation (R) in (a–c). and compared with the control group (normal culture); compared with HBMVECs of OGD/R treated with DMSO in (d).

(a)

(b)

(c)

(d)