Apigenin Protects the Brain against Ischemia/Reperfusion Injury via Caveolin-1/VEGF In Vitro and In Vivo

Figure 2

Apigenin affected cell migration and tube formation of HBMVECs with OGD/R. After OGD/R, lentivirus transfection, and/or treatment with apigenin to HBMVECs, cell migration was measured using the Transwell method (a), and the number of migrating cells stained with crystal violet was harvested and detected at 570 nm for quantitative assessment per filter (b); tube formation of cells was performed and tube networks were quantified as analysis branch points (red arrows indicated) for each field using ImageJ software (e–f). mRNA and protein expression of Caveolin-1 (c–d) and VEGF (g–h) were assayed by qPCR and western blotting, respectively. Data was given as mean ± SD, . compared with HBMVECs transfected by negative lentivirus (HBMVECs + NC) after OGD/R treated with solvent; and compared with HBMVECs + NC after OGD/R treated with apigenin. HBMVECs + KD means HBMVECs transfected by Caveolin-1 knockdown lentivirus.

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