Apigenin Protects the Brain against Ischemia/Reperfusion Injury via Caveolin-1/VEGF In Vitro and In Vivo

Figure 3

Apigenin inhibited apoptosis and autophagy of HBMVEC-combined OGD/R with Caveolin-1. Flow cytometer was used to measure apoptosis and transmission electron microscope to observe autophagy of OGD/R HBMVECs and/or treated with apigenin and intervened with by the Caveolin-1 lentivirus. The associated gene mRNA and protein expression was also assayed by qPCR and western blotting. (a–b) Results of apoptosis and analysis data are shown. mRNA expression of Bcl-2 and Caspase-3 are presented in (c) and (e), and protein expression are shown in (d) and (f). Photographs of cells were taken by transmission electron microscope system, and bilayer or multilayer autophagosome was indicated by red arrows (g). mTOR and Beclin-1 mRNA expression was measured (h, j), and protein expression was analyzed (i, k). Data was given as mean ± SD, . and compared with HBMVECs transfected by negative lentivirus (HBMVECs + NC) after OGD/R treated with solvent; and compared with HBMVECs + NC after OGD/R treated with apigenin. HBMVECs + KD means HBMVECs transfected by Caveolin-1 knockdown lentivirus.

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