Camalexin Induces Apoptosis via the ROS-ER Stress-Mitochondrial Apoptosis Pathway in AML Cells

Figure 5

Camalexin induces ROS generation in leukemia cells. (a) NB4 and HL-60 cells were treated with various doses of camalexin for 12 h, and then intracellular ROS was measured by flow cytometry. (b) NB4 and HL-60 cells were pretreated with or without NAC (20 μM) for 4 h, and then cells were incubated with camalexin (40 μM) for 24 h and cellular apoptosis was measured by flow cytometry. (c) NB4 and HL-60 cells were pretreated with or without NAC (20 μM) for 4 h, and then cells were incubated with camalexin (40 μM) for 48 h and disruption of MMP was measured. (d, e) NB4 and HL-60 cells were pretreated with or without NAC (20 μM) for 4 h, and then cells were incubated with camalexin (40 μM) for 24 h and total cellular lysates were subjected to Western blot analysis with indicated antibodies. (f, g) NB4 and HL-60 cells were treated as described above, and then the release of mitochondrial proteins and activation of Bax were assayed by Western blotting. Mean and SD of three independent experiments performed in triplicate are shown; , , and .

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