The Causal Role of Mitochondrial Dynamics in Regulating Insulin Resistance in Diabetes: Link through Mitochondrial Reactive Oxygen Species

Figure 6

Dynamic proteins modulate mitochondrial ROS in cybrid B4. (a) Cybrid B4 was transfected with plasmid for 24 h to overexpress particular dynamic proteins. (b) Cybrid B4 was transfected with siRNA for 48 h to knockdown particular dynamic proteins. (c) Cybrid B4 was treated with 10 μM mdivi-1 for 24 h to inhibit mitochondrial fission. Mitochondrial ROS production was visualized using MitoSOXRed, which was nuclear counterstained using DAPI. Fluorescent intensity of mitochondrial ROS was quantified using ImageJ. between indicated groups.

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