Palmitate Stimulates the Epithelial Sodium Channel by Elevating Intracellular Calcium, Reactive Oxygen Species, and Phosphoinositide 3-Kinase Activity

Figure 2

Palmitate (PA) stimulates ENaC activity in a dose-dependent manner. (a) Representative ENaC single-channel currents recorded in A6 cells under control conditions or treated with palmitate at concentrations of 1, 10, 50, 300, 500, and 1000 μM. (b) ENaC _O was plotted as a function of each corresponding concentration of palmitate and fitted with pharmacology standard curves. Analysis was performed using SigmaPlot ( for each data point).

(a)

(b)