Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells

Figure 3

(a–c) Effects of cyclic strain on MAPK phosphorylation. Representative Western blots of phosphospecific and total ERK (a), JNK (b), and p38 (c) from cell lysates collected at the indicated times after cyclic strain treatment. Optical density measurements were obtained to determine the relative amounts of phosphorylated MAPK normalized by the respective total MAPK. The values (, ) indicate the fold change in phosphorylation relative to static controls for each individual experiment. indicates a significant difference from the static control (). (d) Effects of cafestol on cyclic-strain-induced phosphorylation of MAPK. Upper panels: Western blots of phospho-ERK, phospho-JNK, and phospho-p38 in HUVECs pretreated with cafestol (10 μM) for 12 h and then treated with cyclic strain for 30 min. Lower panel: quantitative analysis of stretch-induced phosphorylation of MAKP. Results are representative of four individual experiments and expressed as the (). versus untreated controls; versus cells exposed to cyclic strain.

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