Genetically Engineered Resveratrol-Enriched Rice Inhibits Neuroinflammation in Lipopolysaccharide-Activated BV2 Microglia Via Downregulating Mitogen-Activated Protein Kinase-Nuclear Factor Kappa B Signaling Pathway

Figure 8

Treatment with resveratrol-enriched rice inhibits proinflammatory cytokine production in lipopolysaccharide-activated BV2 cells. BV2 microglial cells were pretreated with normal rice (NR), resveratrol-enriched rice (RR), and resveratrol 30 min prior to lipopolysaccharide (LPS; 100 ng/mL) stimulation. Proinflammatory cytokine levels were measured in the conditioned medium of treated cells using ELISA assay after 24 h of LPS activation. The proinflammatory cytokine levels in NR-, RR-, and resveratrol-treated BV2 cells were evaluated. (a) IL-6 production, (b) TNF-α secretion, (c) IL-1β secretion, and (d) PGE2 secretion. All data are presented as the mean ± standard error of the mean of three independent experiments. , , and indicate significant differences compared with LPS treatment alone. indicates significant differences compared with untreated control group. and indicate significant differences in RR compared with NR group. Ctl, untreated control.

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