p-Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress-Induced Apoptosis by MAPK Signaling

Figure 3

p-CA suppressed intracellular ROS production and mitochondrial membrane potential loss in H₂O₂-treated HLE cells. (a) HLE cells were pretreated with p-CA (3, 10, and 30 μM) for 2 h before 275 μM H₂O₂ treatment for 6 h. After staining with H2DCF-DA, cells were incubated at 37°C for 30 min in the darkness and then detected by flow cytometry. (b) HLE cells were preincubated with p-CA (3, 10, and 30 μM) for 2 h before 275 μM H₂O₂ treatment for 6 h. Cells were incubated with Rh123 for 30 min at 37°C, and then the fluorescent intensity was analyzed using flow cytometry. Data are expressed as the mean ± SEM (). , compared with the untreated control group; , , and , compared with the H₂O₂-treated group.

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