p-Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress-Induced Apoptosis by MAPK Signaling

Figure 5

p-CA suppressed MAPK signals in H₂O₂-treated HLE cells. (a) HLE cells were pretreated on p-CA (3, 10, and 30 μM) for 2 h before 275 μM H₂O₂ treatment for 24 h. Cells were lysed, and then p-p-38, p-ERK, and p-JNK expressions were measured using Western blot. (b) HLE cells were pretreated on p-CA (10 μM) or ERK inhibitor (FR180204, 1.25 μM) or p-38 inhibitor (PD169316, 1.25 μM) or JNK inhibitor (SP600125, 1.25 μM) or a combination of both p-CA and SP600125 or PD169316 and FR180204 for 2 h before 275 μM H₂O₂ treatment for 24 h; then the cell viability was measured using the MTT method. Data are expressed as the mean ± SEM (). , compared with the untreated control group; , and , compared with the H₂O₂-treated group; and , compared with the H₂O₂ + p-CA group using Student’s t-test.

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