Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice

Figure 2

Effects of FELDK on AGEs, RAGE, GFAP, and Iba1 expression in the cortex and hippocampus of D-gal-aged mice. (a) AGEs levels were determined by ELISA (). (b) Representative western blots showing protein expression of RAGE, GFAP, and Iba1 (). (c) Quantification of western blot analysis of RAGE, GFAP, and Iba1 protein expressed as percent of control. (d) The immunohistochemical images of active microglia and astrocytes in the cortex and hippocampal dentate gyrus (DG) regions of mice (). Scale bars = 50 μm. All data are presented as mean values ± SEM. , versus the NC group; , versus the D-gal group.

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