Resveratrol Improves Tube Formation in AGE-Induced Late Endothelial Progenitor Cells by Suppressing Syndecan-4 Shedding

Figure 1

Characterization of late EPCs by a light microscope, Western blot, fluorescence microscope, and flow cytometry. (a) MNCs were isolated and plated on a culture dish on the first day. Bar = 100 μm. (b) Morphology of late EPCs in culture 14 days later. Bar = 100 μm. (c) Expressions of eNOS in HUVECs and late EPCs were detected by Western blot, and HUVECs were used as positive controls. (d) Cultured late EPCs stained with DAPI (blue), Dil-acLDL (red), and FITC-Lectin (green). Bar = 10 μm. (e) Late EPCs were identified by representative markers including CD34 and KDR. Bar = 25 μm. (f) Flow cytometric analysis of late EPCs after immunolabelling with CD34, KDR, and CD45.

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