Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes

Figure 2

AHR antagonist CH223191 inhibits ROS production by FICZ. (a) HaCaT cells were treated with DMSO (0.1%, control) or FICZ (1, 10, 100, and 1000 nM) for 6 h or (b) were treated with DMSO (0.1%, control) or FICZ (100 nM) for 1, 2, 4, 6, and 24 h, and the mRNA expression of CYP1A1 was assessed by qRT-PCR. (c) HaCaT cells were treated with DMSO (0.1%, control), BaP (1 μM), or FICZ (100 nM) alone or simultaneously with the AHR antagonist CH223191 (10 μM) for 6 h, and ROS production was assessed by flow cytometry. Mean fluorescence intensities of DCF are shown. Data are presented as means ± standard deviation ( per group). , .

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