Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes

Figure 3

Knockdown of AHR by siRNA inhibits ROS production by FICZ. Negative control siRNA or AHR siRNA was transfected into HaCaT cells, and the efficiency of knockdown of (a) AHR mRNA and (b) AHR protein was determined by qRT-PCR and western blotting, respectively. (c) Cells transfected with siRNA were further treated with DMSO (0.1%, control), BaP (1 μM), or FICZ (100 nM) for 6 h. The effects of AHR knockdown on ROS production were assessed by flow cytometry. Data are presented as means ± standard deviation ( per group). , , .

(a)

(b)

(c)