Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes

Figure 4

FICZ induces the expression of mRNAs of proinflammatory cytokines. HaCaT cells were treated with DMSO (0.1%, control) or FICZ (100 nM) for 3 or 6 h, and the expression of (a) IL1A, (b) IL1B, and (c) IL6 was measured by qRT-PCR. ACTB served as an internal control, and relative expression levels compared with those of DMSO-treated control samples were calculated by using the comparative Ct method. Data are presented as means ± standard deviation ( per group). , .

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