Tryptophan Photoproduct FICZ Upregulates IL1A, IL1B, and IL6 Expression via Oxidative Stress in Keratinocytes

Figure 5

FICZ induces the expression of proinflammatory cytokine genes in a ROS- and AHR-dependent manner. (a) HaCaT cells were treated with DMSO (0.1%, control) or with FICZ (100 nM) in the presence or absence of NAC (5 mM) for 6 h, and ROS production was assessed by flow cytometry. Representative histograms of DCF fluorescence (A) are shown, along with the mean fluorescence intensities of DCF (ROS production) (B). Black: DMSO (0.1%, negative control); orange: FICZ (100 nM); green: FICZ (100 nM) plus NAC (5 mM). (b) HaCaT cells were treated with DMSO (0.1%, control) or FICZ (100 nM) in the presence or absence of NAC (5 mM) for 3 h. Expression of IL1A, IL1B, and IL6 was measured by qRT-PCR. ACTB served as an internal control, and relative expression compared with those of DMSO-treated control samples was calculated by using the comparative Ct method. Data are presented as means ± standard deviation ( per group). , , .

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