ALDH2 Activity Reduces Mitochondrial Oxygen Reserve Capacity in Endothelial Cells and Induces Senescence Properties

Figure 6

ALDH2 silencing affects the HUVEC mitochondrial ultrastructure. Cells were transfected with siRNA as described above. Then, they were harvested and seeded 24 h posttransfection and treated as described in Materials and Methods. TEM representative images of siCTR or siALDH2: (a, c) siCTR and (b, d) siALDH2. M: mitochondria. Scale bar: (a, b) 1 μm and (c, d) 500 nm. Images are representative of results obtained with siALDH2 B.

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