Label-Free Proteomics of the Fetal Pancreas Identifies Deficits in the Peroxisome in Rats with Intrauterine Growth Restriction

Figure 2

Global protein expression patterns in the rat fetal pancreas. (a) Venn diagram showing 1,497 proteins identified in both groups (overlap), 36 proteins detected only in the control group (blue), and 64 proteins detected only in the IUGR group (yellow). (b) Principal component analysis (PCA) graph showing a clear separation between control and IUGR samples. Each point in the PCA graph represents the protein profile of one biological replicate sample. (c) -means clustering representation of the profiles of 57 differentially expressed proteins. The percentage variation is represented by a color scale (top right) from low (blue) to high (red). (d) Volcano plots of the 1,497 quantified proteins showing the distribution of significance and fold change of identified proteins (the logarithmic ratio of protein LFQ intensities in the IUGR/CON comparison was plotted against negative logarithmic values of the -test). Vertical dotted lines mark a fold change of ±150%, and horizontal dotted lines mark a .

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