Label-Free Proteomics of the Fetal Pancreas Identifies Deficits in the Peroxisome in Rats with Intrauterine Growth Restriction

Figure 7

Investigation of peroxisomal-related proteins in the fetus. (a) Western blot analysis of peroxisome-related factors in the fetal pancreas. (b) Densitometry analysis results were expressed as the . , vs. control. (c) The mRNA levels of focus proteins in the fetal pancreas were determined by quantitative RT-PCR with β-actin as the reference gene. (d) Representative photomicrographs of immunofluorescence analysis of Pex14 in sections of the pancreas from the control (A, B, C) and IUGR (D, E, F) (original magnification 400x). (e) The STRING network of peroxins and peroxisomal FAO with altered expression. Colored lines between the proteins indicate the various types of interaction evidence. (f) Schematic representation of the peroxisomal FAO, which consists of 4 steps catalyzed by different enzymes and leading to the formation of a chain-shortened acyl-CoA and acetyl-CoA.

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