Label-Free Proteomics of the Fetal Pancreas Identifies Deficits in the Peroxisome in Rats with Intrauterine Growth Restriction
Table 1
Proteins chosen for validation.
Protein ID
Gene symbol
Proteins
Unique peptides
Average iTRAQ ratio ()
value
Main function
Q642G4
Peroxisomal membrane protein Pex14
1
1
0.2805
0.011
An essential component of the peroxisomal import machinery. Plays a key role for peroxisome movement through a direct interaction with tubulin.
Q6IN39
Peroxisomal multifunctional enzyme type 2 Hsd17b4
2
6
0.5702
0.05
Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids.
D3ZVU7
Histone deacetylase1
3
3
0.4278
0.0053
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4).
P39951
Cyclin-dependent kinase 1
19
3
0.5939
0.0223
Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset
D3ZP96
DNA helicase MCM2
1
3
0.5621
0.0033
A component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for “once per cell cycle” DNA replication initiation and elongation in eukaryotic cells. Plays a role in cell apoptosis.
B2GUX3
DNA helicase MCM5
3
6
2.0485
0.0283
A component of the MCM2-7 complex. Interacts with MCMBP.
Have/no
D3ZGX8
Bromodomain-containing 4
2
1
None
Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation.
F1LQB2
Structural maintenance of chromosomes protein Smc3
3
5
None
Central component of cohesin, a complex required for chromosome cohesion during the cell cycle.
Q08420
Extracellular superoxide dismutase [Cu-Zn]SOD3
1
1
Have
Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
C: control; I: IUGR; peptide: the number of peptides identified by LC-MS/MS.
