Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction

Figure 3

Inhibiting HMGB1 by GL relieved BMSC dysfunction under HG condition. (a) Cell viability evaluated by the CCK-8 assay at 3 d, 7 d, and 14 d, . (b) ALP staining of BMSCs after an osteogenic induction of 3 d, 7 d, and 14 d. (c) Alizarin red staining of BMSCs after an osteogenic induction of 21 days and deposited calcium stains red. (d) Quantification of mineralization nodules in different groups, . Relative expressions of Runx2 (e), ALP (f), OCN (g), and RANKL/OPG (h) detected at 3 d, 7 d, and 14 d by RT-qPCR analysis, . Representative band (i) and quantification (j) of protein levels of OPG and RANKL at 14 d, . All data were normalized to β-actin. , ; .

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