Inhibition of HMGB1 Promotes Osseointegration under Hyperglycemic Condition through Improvement of BMSC Dysfunction

Figure 7

Inhibiting HMGB1 rescued impaired osseointegration in diabetic rats. (a) Quantification of BV/TV, Tb.N, Tb.Th, and Tb.Sp within the VOI. (b) Morphology of peri-implant trabeculae. Four weeks after implantation, the trabecular thickness and number were quite similar in three groups. After another healing period of 4 weeks, however, the trabeculae became sparser and thinner among rats in the diabetic group. While in the control group, the trabeculae got denser and well organized. In the diabetic-GL group, the reduction in trabecular number caused by diabetes was relieved, but not eliminated. (c) Morphology of peri-implant trabecular separation. In the diabetic group, the intertrabecular space is filled with macro yellow-to-red bubbles, indicating large space and big separation. While in the control group, the space is filled with micro green-to-blue bubbles, representing thin space and small separation. (d) Representative images of BIC by μCT evaluation. The pink area represents bone in direct contact with the implant. (e) Quantification of BIC by μCT evaluation. Representative images (f) and quantification (g, h) of BIC under histomorphometry, showing the newly formed bone tissue adjacent to the implant surface (Masson trichrome staining). . , ; .

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