Verapamil Attenuated Prediabetic Neuropathy in High-Fat Diet-Fed Mice through Inhibiting TXNIP-Mediated Apoptosis and Inflammation

Figure 3

Effects of verapamil on nerve conduction velocity and protein expressions related to metabolism and inflammation in high-fat diet-fed mice. (a) The MNCV and SNCV were detected. After HF mice exhibited IGT for the first time, four groups of mice were anesthetized, and MNCV and SNCV were measured for 3 times with an interval of 15 min. Each value represents mean ± SEM (). ; . (b) The expressions of TXNIP, NLRP3, caspase-1, and IL-1β were detected. The sciatic nerve was isolated, and the protein expressions in sciatic nerve including TXNIP, NLRP3, caspase-1, and IL-1β were determined by western blotting analysis, and the target protein expressions relative to GAPDH expression were displayed on the right. The experiments were performed in triplicate, and each value represents mean ± SD. ; ; .

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