Research Article
Intramelanocytic Acidification Plays a Role in the Antimelanogenic and Antioxidative Properties of Vitamin C and Its Derivatives
Figure 7
Detection of ROS level by fluorescence microscopy and flow cytometry analysis using DCFH-DA labeling. MCs were exposed to 3 J/cm2 UVA for 24 h and were then treated with 1 mM VC for an additional 24 h. Untreated or treated cells were loaded with 10 μM DCFH-DA in serum-free medium for 30 min in the dark. The labeled MCs were observed using a fluorescence microscope (a) and were (b) quantitatively analyzed using a BD FACSCalibur flow cytometer. Scale bar: 50 μm.
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