TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response

Figure 1

Expression and secretion of TNFα are increased in the fatty liver after IRI. (a) Representative H&E staining of liver sections of CD- and HFD-fed mice after IR. Scale bars, 200 μm. (b) Serum ALT of CD- and HFD-fed mice after IR were measured (). (c) qPCR analysis of TNFα of CD- and HFD-fed mice after IR ( per group). (d) Serum TNFα was measured after IR in CD- and HFD-fed mice ( per group). (e) Representative Oil Red O staining of KCs treated with PA (500 μM, Sigma-Aldrich, USA) or PBS for 24 h. Scale bars, 100 μm. (f) TNFα in cell supernatant were measured ( per group). (g) qPCR analysis of TNFα in KCs treated with PA followed by H/R. Data are ; , , , and by unpaired Student’s -test.

(a)

(b)

(c)

(d)

(e)

(f)

(g)