TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response

Figure 2

TNFα induces necroptosis in vitro. Hepatocytes were cultured with TNFα (PeproTech, USA) or cell supernatant of KCs treated with PA followed by H/R for 24 h. (a) Immunoblot analysis of RIP1, RIP3, and MLKL of hepatocytes treated with different concentrations of TNFα. (b) Immunoblot analysis of RIP1, RIP3, and MLKL of hepatocytes treated with cell supernatant of KCs for 24 h. (c, d) Representative immunofluorescence staining of propidium iodide (PI) staining of hepatocytes treated with TNFα (20 ng/mL) for 24 h. Scale bars, 100 μm. (e, f) Representative immunofluorescence staining of PI of hepatocytes treated with cell supernatant of KCs for 24 h. Scale bars, 100 μm. Data are ; , , and by unpaired Student’s -test.

(a)

(b)

(c)

(d)

(e)

(f)