TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response

Figure 3

Necroptosis is found in NAFLD after IRI. (a) Immunoblot analysis of necroptosis markers RIP1, RIP3, and MLKL of mice fed with a CD or a HFD. (b) Immunoblot analysis of RIP1, RIP3, and MLKL of CD- and HFD-fed mice after 60 min of ischemia and 6 h of reperfusion. (c) Representative immunofluorescence staining of RIP3 was performed in CD- and HFD-fed mice with or without 60 min of ischemia and 6 h of reperfusion. Scale bars, 100 μm.

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