TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response

Figure 4

Inhibition of necroptosis reduces ischemia-reperfusion injury of NAFLD. (a) Immunoblot analysis of necroptosis markers RIP1, RIP3, and MLKL of mice fed with a CD after IRI pretreated with Nec-1 (1.65 mg/kg, Selleck, USA), GSK872 (1.9 mM/kg, Selleck, USA), and TN3-19.12 (250 μg/mouse, Sigma-Aldrich, USA). (b) Immunoblot analysis of necroptosis markers RIP1, RIP3, and MLKL of mice fed with HFD after IRI pretreated with TN3-19.12, Nec-1, and GSK872. (c, d) Serum ALT and AST of mice fed with a CD after IRI ( per group). (e, f) Serum ALT and AST of mice fed with a HFD after IRI ( per group). (g) Representative H&E staining of liver sections. Scale bars, 200 μm. (h) Representative immunofluorescence staining of PI. Scale bars, 100 μm. Data are ; by unpaired Student’s -test.

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