TNFα-Mediated Necroptosis Aggravates Ischemia-Reperfusion Injury in the Fatty Liver by Regulating the Inflammatory Response

Figure 5

Inhibition of necroptosis reduces hepatocyte death induced by TNFα in vitro. (a) Hepatocytes were cultured with TNFα (20 ng/mL) for 24 h. Immunoblot analysis of necroptosis markers RIP1, RIP3, and MLKL of hepatocytes pretreated with Nec-1 (100 μM) and GSK872 (5 μM). (b) Representative immunofluorescence staining of PI. Scale bars, 100 μm.

(a)

(b)